Subcomandante wrote: ↑November 29th, 2021, 3:36 pm
The mRNA technology is the only technology that uses the spike proteins. That in itself is concerning.
...mRNA is just the type of genetic programming for the body to create spike proteins...the genetic therapy to make the human body a spike protein factory is not limited to mRNA "vaccines"...the "mRNA technology doesn't USE the spike proteins"...it is instructions for the body to manufacture them...
The Johnson & Johnson vaccine is slightly different. It uses double-stranded DNA inserted into a common, but inert virus called an adenovirus. This DNA also contains the instructions for building the spike protein. Once inside the cell, these instructions are read and translated into mRNA.
The mRNA chains are basically work orders that spell out the instructions for making the spike proteins that stud the outside of the coronavirus that cases COVID-19.
https://www.webmd.com/lung/news/2021071 ... ne-therapy
...that's from mainstream....
Messenger RNA (mRNA) is a single-stranded RNA molecule that is complementary to one of the DNA strands of a gene. The mRNA is an RNA version of the gene that leaves the cell nucleus and moves to the cytoplasm where proteins are made. During protein synthesis, an organelle called a ribosome moves along the mRNA, reads its base sequence, and uses the genetic code to translate each three-base triplet, or codon, into its corresponding amino acid.
https://www.genome.gov/genetics-glossary/messenger-rna
Once the mRNA arrives, the cell can produce particular proteins from these instructions.
https://www.civilbeat.org/2021/04/what- ... t-it-does/
By injecting cells with a synthetic mRNA that encodes a viral spike protein, an mRNA vaccine can direct human cells to make a viral spike protein and evoke an immune response without a person ever having been exposed to the viral material.
https://www.genome.gov/about-genomics/f ... A-Vaccines
This chapter describes our current understanding of the mechanisms involved in coronavirus mRNA translation and changes in host mRNA translation observed in coronavirus-infected cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5388242/
...the technology encodes the same full sequence spike protein that the wild caught virus utilizes...but they took some shortcuts...
Vaccine RQ3012-Spike encodes the full-length wild-type S, while RQ3013-VLP is formulated from a cocktail of mRNAs encoding three structural proteins: S, M (membrane), and E (envelope) to form SARS-CoV-2 virus-like particles (VLPs). To increase the expression capacity of mRNA vaccines, all mRNAs were subjected to an in-depth sequence optimization procedure of two parameters: codons in the DNA template and modified nucleotides incorporated into mRNA. We designed ten coding sequences of the S gene (3822 nucleotides in length) with varying GC-content, maintaining the maximum codon adaptation index.
https://www.nature.com/articles/s41422-020-00392-7
CureVac AG discovered that human mRNA codons rarely have A or U at the third position and patented a strategy that replaces A or U at the third position in the open reading frame with G or C21.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8386155/
The two most frequently used SARS-CoV-2 vaccines, from Pfizer/BioNTech [1] and Moderna [2], respectively, are both mRNA vaccines. The sequence of Pfizer/BioNTech’s BNT-162b2 is publicly available [3], and the sequence of Moderna’s mRNA-1273 has recently been sequenced [4]. Both mRNA encodes the same S-2P protein [5,6] which differ from the spike protein in the reference SARS-CoV-2 genome (NC_045512) by two amino acids, i.e., amino acids KV at sites 986 and 987 were replaced by PP to stabilize the resulting spike protein in the prefusion state to train the host immune system to recognize the virus before its entry into the host cell [7,8].
While the Pfizer/BioNTech’s BNT162b2 mRNA and Moderna’s mRNA-1273 share the same amino acid sequence, they differ in many other ways, such as the design of 5′-UTR, codon optimization and 3′-UTR. Translation initiation is typically the limiting step in translation, and its efficiency depends heavily on how rapidly the 5′-UTR can load ribosome onto the mRNA [9]. Translation elongation becomes rate-limiting when translation initiation is highly efficient [10,11]. Optimization of vaccine mRNA for efficient translation can decrease the copies of vaccine mRNA needed to be carried into host cells.
...and the spike protein is toxic all by itself...so getting vaccinated is getting genetic programming for the body to manufacture a toxin (spike protein)...
other papers showed that the spike protein by itself (without being part of the corona virus) can damage endothelial cells and disrupt the blood-brain barrier.
https://pubmed.ncbi.nlm.nih.gov/34100279/
...and they make claims about what can and can't happen...but they don't know for sure...
Even if it could get into the nucleus, the single strand of mRNA would have to be translated back into a double stranded DNA.
HIV, the virus that causes AIDS, can do this. It uses an enzyme like reverse transcriptase to insert itself into our chromosomes. The mRNA in the vaccines lacks this enzyme, so it can't turn back into DNA.
The DNA adenovirus used in the Johnson & Johnson vaccine does enter the nucleus of our cells, but it never integrates into our chromosomes.
https://www.webmd.com/lung/news/2021071 ... ne-therapy
The first U in the stop codon in translating AZ1 mRNA is often skipped when the concentration of polyamines is high, resulting in the ribosome reading GAU as the next codon [85]. With such a +1 frameshifting, a downstream in-frame stop codon cannot serve as a fail-safe mechanism. UGA is a poor choice of a stop codon, and UGAU in Pfizer/BioNTech and Moderna mRNA vaccines could be even worse.
As Ψ is more promiscuous in base-pairing than U and can pair with both A and G and, to a less extent, with C and U [16], stop codons become more prone to misreading by tRNAs [17]. It is for this reason that both mRNA vaccines use consecutive stop codons as a fail-safe mechanism, with the hope that no frameshifting occurs when the first stop codon fails. However, UGAU is known to cause a +1 frameshifting. It is reasonable to infer that ΨGAΨ may be the same.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8310186/
Codon usage is an important determinant of gene expression levels largely through its effects on transcription
https://www.pnas.org/content/113/41/E6117
...more of a currently held belief....that we'll be able in a decade or so to address more fully...
Codon bias has been implicated as one of the major factors contributing to mRNA stability in several model organisms. However, the molecular mechanisms of codon bias on mRNA stability remain unclear in humans.
https://www.embopress.org/doi/full/10.1 ... .201948220
The RNA sequence of the vaccine as well as the spike protein target interaction were analyzed for the potential to convert intracellular RNA binding proteins TAR DNA binding protein (TDP-43) and Fused in Sarcoma (FUS) into their pathologic prion conformations. The results indicate that the vaccine RNA has specific sequences
that may induce TDP-43 and FUS to fold into their pathologic prion confirmations
Furthermore, the spike protein, created by the translation of the vaccine RNA, binds angiotensin converting enzyme 2 (ACE2), a zinc containing enzyme. This interaction has the potential to increase intracellular zinc. Zinc ions have been shown to cause the transformation of TDP-43 to its pathologic prion configuration. The folding of TDP-43 and FUS into their pathologic prion confirmations is known to cause ALS, front temporal lobar degeneration, Alzheimer’s disease and other neurological degenerative diseases.
https://dundasvalley.files.wordpress.co ... e-1503.pdf
Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
https://www.pnas.org/content/118/21/e2105968118
....so to summarize...courtesy of Dr. Mercola...
mRNA-based COVID shots have used codon optimization to improve protein production. A codon consists of three nucleotides, and nucleotides are the building blocks of DNA. Use of codon optimization virtually guarantees unexpected results
Replacing rare codons must be done judiciously, as rarer codons can have slower translation rates and a slowed-down rate is actually necessary to prevent protein misfolding
Stop codons, when present at the end of an mRNA coding sequence, signals the termination of protein synthesis. According to a recent paper, both Pfizer and Moderna selected suboptimal stop codons
The COVID shots induce spike protein at levels unheard of in nature, and the spike protein is the toxic part of the virus responsible for the most unique effects of the virus, such as the blood clotting disorders, neurological problems and heart damage. To expect the COVID shot to not produce these kinds of effects would be rather naïve
Other significant threats include immune dysfunction and the flare-up of latent viral infections such as herpes and shingles. Coinfections, in turn, could accelerate other diseases. Herpes viruses, for example, have been implicated as a cause of both AIDS and chronic fatigue syndrome
https://www.nutritruth.org/single-post/ ... king-about